Citrus MAF1, a Repressor of RNA Polymerase III, Binds the Xanthomonas citri Canker Elicitor PthA4 and Suppresses Citrus Canker Development
Identifieur interne : 001134 ( Main/Exploration ); précédent : 001133; suivant : 001135Citrus MAF1, a Repressor of RNA Polymerase III, Binds the Xanthomonas citri Canker Elicitor PthA4 and Suppresses Citrus Canker Development
Auteurs : Adriana Santos Soprano [Brésil] ; Valeria Yukari Abe [Brésil] ; Juliana Helena Costa Smetana [Brésil] ; Celso Eduardo Benedetti [Brésil]Source :
- Plant physiology : (Bethesda) [ 0032-0889 ] ; 2013.
Descripteurs français
- Pascal (Inist)
- Wicri :
- topic : Répression, Agrume.
English descriptors
- KwdEn :
Abstract
Transcription activator-like (TAL) effectors from Xanthomonas species pathogens act as transcription factors in plant cells; however, how TAL effectors activate host transcription is unknown. We found previously that TAL effectors of the citrus canker pathogen Xanthomonas citri, known as PthAs, bind the carboxyl-terminal domain of the sweet orange (Citrus sinensis) RNA polymerase II (Pol II) and inhibit the activity of CsCYP, a cyclophilin associated with the carboxyl-terminal domain of the citrus RNA Pol II that functions as a negative regulator of cell growth. Here, we show that PthA4 specifically interacted with the sweet orange MAF1 (CsMAF1) protein, an RNA polymerase III (Pol III) repressor that controls ribosome biogenesis and cell growth in yeast (Saccharomyces cerevisiae) and human. CsMAF1 bound the human RNA Pol III and rescued the yeast maf1 mutant by repressing tRNAHis transcription. The expression of PthA4 in the maf1 mutant slightly restored tRNAHis synthesis, indicating that PthA4 counteracts CsMAF1 activity. In addition, we show that sweet orange RNA interference plants with reduced CsMAF1 levels displayed a dramatic increase in tRNA transcription and a marked phenotype of cell proliferation during canker formation. Conversely, CsMAF1 overexpression was detrimental to seedling growth, inhibited tRNA synthesis, and attenuated canker development. Furthermore, we found that PthA4 is required to elicit cankers in sweet orange leaves and that depletion of CsMAF1 in X. citri-infected tissues correlates with the development of hyperplastic lesions and the presence of PthA4. Considering that CsMAF1 and CsCYP function as canker suppressors in sweet orange, our data indicate that TAL effectors from X. citri target negative regulators of RNA Pol II and Pol III to coordinately increase the transcription of host genes involved in ribosome biogenesis and cell proliferation.
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aut.</sZ><sZ>4 aut.</sZ></inist:fA14><country>Brésil</country><placeName><settlement type="city">São Paulo</settlement><region type="state">État de São Paulo</region></placeName></affiliation></author><author><name sortKey="Yukari Abe, Valeria" sort="Yukari Abe, Valeria" uniqKey="Yukari Abe V" first="Valeria" last="Yukari Abe">Valeria Yukari Abe</name><affiliation wicri:level="3"><inist:fA14 i1="01"><s1>Laboratório Nacional de Biociências, Centro Nacional de Pesquisa em Energia e Materials</s1><s2>CEP 13083-970, Campinas, Sao Paulo</s2><s3>BRA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ></inist:fA14><country>Brésil</country><placeName><settlement type="city">São Paulo</settlement><region type="state">État de São Paulo</region></placeName></affiliation></author><author><name sortKey="Costa Smetana, Juliana Helena" sort="Costa Smetana, Juliana Helena" uniqKey="Costa Smetana J" first="Juliana Helena" last="Costa Smetana">Juliana Helena Costa Smetana</name><affiliation wicri:level="3"><inist:fA14 i1="01"><s1>Laboratório Nacional de Biociências, Centro Nacional de Pesquisa em Energia e Materials</s1><s2>CEP 13083-970, Campinas, Sao Paulo</s2><s3>BRA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ></inist:fA14><country>Brésil</country><placeName><settlement type="city">São Paulo</settlement><region type="state">État de São Paulo</region></placeName></affiliation></author><author><name sortKey="Eduardo Benedetti, Celso" sort="Eduardo Benedetti, Celso" uniqKey="Eduardo Benedetti C" first="Celso" last="Eduardo Benedetti">Celso Eduardo Benedetti</name><affiliation wicri:level="3"><inist:fA14 i1="01"><s1>Laboratório Nacional de Biociências, Centro Nacional de Pesquisa em Energia e Materials</s1><s2>CEP 13083-970, Campinas, Sao Paulo</s2><s3>BRA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ></inist:fA14><country>Brésil</country><placeName><settlement type="city">São Paulo</settlement><region type="state">État de São Paulo</region></placeName></affiliation></author></analytic><series><title level="j" type="main">Plant physiology : (Bethesda)</title><title level="j" type="abbreviated">Plant physiol. : (Bethesda)</title><idno type="ISSN">0032-0889</idno><imprint><date when="2013">2013</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Plant physiology : (Bethesda)</title><title level="j" type="abbreviated">Plant physiol. : (Bethesda)</title><idno type="ISSN">0032-0889</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Bacteriosis</term><term>Citrus</term><term>Citrus fruit</term><term>DNA-directed RNA polymerase</term><term>Development</term><term>Elicitor</term><term>Plant physiology</term><term>Repression</term><term>Xanthomonas campestris pv. citri</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Répression</term><term>Eliciteur</term><term>Bactériose</term><term>Développement</term><term>Citrus</term><term>Xanthomonas campestris pv. citri</term><term>Agrume</term><term>DNA-directed RNA polymerase</term><term>Physiologie végétale</term></keywords><keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Répression</term><term>Agrume</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Transcription activator-like (TAL) effectors from Xanthomonas species pathogens act as transcription factors in plant cells; however, how TAL effectors activate host transcription is unknown. We found previously that TAL effectors of the citrus canker pathogen Xanthomonas citri, known as PthAs, bind the carboxyl-terminal domain of the sweet orange (Citrus sinensis) RNA polymerase II (Pol II) and inhibit the activity of CsCYP, a cyclophilin associated with the carboxyl-terminal domain of the citrus RNA Pol II that functions as a negative regulator of cell growth. Here, we show that PthA4 specifically interacted with the sweet orange MAF1 (CsMAF1) protein, an RNA polymerase III (Pol III) repressor that controls ribosome biogenesis and cell growth in yeast (Saccharomyces cerevisiae) and human. CsMAF1 bound the human RNA Pol III and rescued the yeast maf1 mutant by repressing tRNA<sup>His</sup> transcription. The expression of PthA4 in the maf1 mutant slightly restored tRNA<sup>His</sup> synthesis, indicating that PthA4 counteracts CsMAF1 activity. In addition, we show that sweet orange RNA interference plants with reduced CsMAF1 levels displayed a dramatic increase in tRNA transcription and a marked phenotype of cell proliferation during canker formation. Conversely, CsMAF1 overexpression was detrimental to seedling growth, inhibited tRNA synthesis, and attenuated canker development. Furthermore, we found that PthA4 is required to elicit cankers in sweet orange leaves and that depletion of CsMAF1 in X. citri-infected tissues correlates with the development of hyperplastic lesions and the presence of PthA4. Considering that CsMAF1 and CsCYP function as canker suppressors in sweet orange, our data indicate that TAL effectors from X. citri target negative regulators of RNA Pol II and Pol III to coordinately increase the transcription of host genes involved in ribosome biogenesis and cell proliferation.</div></front></TEI><affiliations><list><country><li>Brésil</li></country><region><li>État de São Paulo</li></region><settlement><li>São Paulo</li></settlement></list><tree><country name="Brésil"><region name="État de São Paulo"><name sortKey="Santos Soprano, Adriana" sort="Santos Soprano, Adriana" uniqKey="Santos Soprano A" first="Adriana" last="Santos Soprano">Adriana Santos Soprano</name></region><name sortKey="Costa Smetana, Juliana Helena" sort="Costa Smetana, Juliana Helena" uniqKey="Costa Smetana J" first="Juliana Helena" last="Costa Smetana">Juliana Helena Costa Smetana</name><name sortKey="Eduardo Benedetti, Celso" sort="Eduardo Benedetti, Celso" uniqKey="Eduardo Benedetti C" first="Celso" last="Eduardo Benedetti">Celso Eduardo Benedetti</name><name sortKey="Yukari Abe, Valeria" sort="Yukari Abe, Valeria" uniqKey="Yukari Abe V" first="Valeria" last="Yukari Abe">Valeria Yukari Abe</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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